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What is chiral and why this performing in HPLC
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Answer Posted / rajasekhar
Some samples have chiral centers . These samples cant saparate reverse phase columns and Narmal phase columns. We can saparate these sample only Chiral columns.Due to charal samples have Isomers, these isomers have same polarity and phisical property.
In chiral columns have one Isomer , which interacts with sample isomers differently , thats why we can saparate.
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