what is method development parameter in HPLC method
development
Answer Posted / sivannarayana
1.Solubility
2.Find out the UV scan For wave length
3.optimaize Buffer & pH Value Optimaize
4.Optimaize Column Temparature & optimaize sample cooler Temparature.
5.optimaize the GP For better Resolution
| Is This Answer Correct ? | 4 Yes | 0 No |
Post New Answer View All Answers
in hplc chromatogram started from left to right and in uv spectrum started from left to right
what you do when compound having dwel wavelength?
If we have 5 strength which is not dose proportinate and excipients also diffrent in each strength then how we can proceed for Force degradation? and excipient are same but not dose propotinate the how FD?
What is third generation HPLC Columns?
we are performed SOR for a particular product the limit is -6 to -10.We face particular bathes it is not meeting the specification.we performed diffrent instrument diffrent analyst we are getting diffrent diffrent results.solvent is dimethyl sulfoxide what could be the reasion
Why irmoisture balance is used for corrugated box moisture testing
why octanol used to determine the partition coefficient ?
what is mean by ambient temperature?
what is lod and loq ?,why use k2cr2o7 , kcl h2so4 in uv calibration ?,why use benzophenone & caffene acetone in hplc calibration ?,what is leading peak in hplc ?why we do the calibration of limit of stry light in hplc & uv ?
when we talk about change in entropy we consider total entropy means of surround +entropy of system but when we talk about change in Gibss free energy we consider entropy of system.explain with reason.
If we do accuracy at same concentration at which linearity planned,what is the need to do linearity separately?
How do we fix the sample concentaryion in hplc method development?
how to set sample and standard concentration in RS method?
Why only Copper standard is used to calibrate Atomic Absorption spectrophotometer?
AT WHAT CONDITIONS WILL YOU ANALYSE A SAMPLE,WHICH ARE PRESERVED AT OTHER THAN THE AMBIENT CONDITIONS?(ie COLD STORAGE SAMPLES BELOW 20* CENTIGRADE?