Interested to Buy Any Domain ? << Click Here >> for more details...
If i performing one analysis on C8column then the same analysis done on C18 column then what will happen about Rt;Resulution;Peak shape Explain ?
- 1 Answers
- 8105 Views
- I also Faced
- E-Mail Answers
Answer Posted / t reddy
consider ur performing combination drug.... if both the drugs hydrophilic means.
in c8 column the rt will be high compare to c18 and resolution between two peaks also will increase in c8.
i think peak shape won't affect based on this factor.
Reason - c8 is less hydrophobic than c18... because of less carbon.... c18 is more hydrophobic so the interaction between hydrophilic drug and high hydrophobic column(c18) is less, drug will elute quickly and RT will be less than c8 column and resolution also.
NOTE - this is just a possibility, u can still get the good Rt with c8 also by changing the various factors like mobile phase composition, flow rate, temperature etc
| Is This Answer Correct ? | 2 Yes | 1 No |
Post New Answer View All Answers
If vendor having more imp than monograph then how to proceed? and how to set spec?
why salisylic acid not using now days for disdolution calibration?
we are performed SOR for a particular product the limit is -6 to -10.We face particular bathes it is not meeting the specification.we performed diffrent instrument diffrent analyst we are getting diffrent diffrent results.solvent is dimethyl sulfoxide what could be the reasion
What is the principle of Thermal conductivity detector and FID?
what is mean by 40 in the dissolution basket mesh size?
what is third integration
why lactose has less charges when packed in steel containers when compared with packing of lactose in polyamide material?
mode of absorption in alimentary canal?
How they found 1mL of K.F reagent is equivalent to 5mg of water and if we change the composition of K.F reagent, is it can neutralize more amount of water?
What is the difference between purge septum flow and column flow in gas chromatography?
Principle of single pan analytical balance
How to establish relative response factor for hplc. Why it is required?
on saturation solubility study data how we can find out the bcs class of drug?
Why we check moisture,ash & AIA in product
using gradient pressure in gas chromatography are not ?using gradient pressure why
