HPLC Calibration was done in Reverse Phase, but not in
normal phase why explain?
Answer Posted / zahir
In reverse phase it is easy to calibrate hplc than normal phase bec in normal phase n haxane is used it v highly evapurate so it also change in the area of the sample as time passes and n hexane is break septa of vial so we preferred reverse phase than normal phase.
| Is This Answer Correct ? | 0 Yes | 4 No |
Post New Answer View All Answers
We use hicrome 10 cxs column in hplc analysis....pls tell me.. what is the meaning of 10 cxs?
Why are use silicon oil mr?
What is stray light in uv what are units for stray light
How can we confirm the HPLC column is end-capped or not? Is it possible to identify by physical appearance?
some product having water content method with pyridine and ethylene glycol mixture instead of methanol? how they are selecting and methanol is not suitable solvent how ?
[3/28, 20:52] Manoj P Venkatpurwar: how many impurities require to inject in assay specificity that how we can find out? and in Rs also how?
Why to use propyl paraben in HPLC gradient calibration
what are the guidelines for analytical method validations?
why glutent are detected in the rice cereal baby food product even manufacturer claimed that they are using rice and milk only?we have using ELISA to do the test,and rice supposed not containing any glutent,rite?We already repeat the test so many times and it still detected.just wondering where the glutent came from?
How we choose the mobile phase for method development.?
if you have given one product then how you determine the impurity in that?
How to choose the which salt is suitable for mobilephase
in stress study if your api not soluble in hcl naoh h2o2 then what require to do?
What is the formula for relative diffrence for standard solution in solution stability in validation?
we can use expired sample for validation and analyst qualification?