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HPLC Calibration was done in Reverse Phase, but not in
normal phase why explain?
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Answer Posted / zahir
In reverse phase it is easy to calibrate hplc than normal phase bec in normal phase n haxane is used it v highly evapurate so it also change in the area of the sample as time passes and n hexane is break septa of vial so we preferred reverse phase than normal phase.
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