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I got 88.0 % assay for a drug in HPLC analysis for same
injection i got 40.0% related substances is it possible?
what is the relation between results of assay and purity?

Answer Posted / a.prabhakar

May it is possible above case. Related substances is qualitative test but purity/assay is quantitative test.In quantitative test sample area compare with standard area.

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