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Why we use caffeine for HPLC calibration in Detector wave
ength accuracy test
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Answer Posted / manikyam
It will give maxima at 273nm and 205nm and also give minima at 245nm. caffeine fastely ellute in any mobilephase like water or methanol.
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2. Two grams of Benzoic acid are dissolved in 200 ml of water and extracted with 200 ml of diethyl ether. The distribution coefficient of benzoic acid is 100, and its dissociation constant is 6.5 10-5. Calculate the distribution ratio (D) of benzoic acid at pH 2, 5, and 6. 3. Calculate D at pH 2 to 10 (1 unit apart) in the above problem, and plot D versus pH.
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