why do we adjusted ph in the mobilphase
Answer Posted / simhadri
It is better to adjust our mobile PH minium 1 or 2 units away from the analyte PKa,to get good peak shape and good retention.
Is This Answer Correct ? | 8 Yes | 1 No |
Post New Answer View All Answers
Difference between the quantitative analysis and qualitative analysis?
how you confirm the assay method?
what is the origin to prepare standard operating procedure
How to know the estimated LOQ concentration in ppm
Why we use potassium dichromate in uv calibration Exact reason behind it??
what is different when impergnated silica plates are used in separation of azo dyes using column chromatography?
in which situation ion pair require to use?
1.What is the difference between method validation and method verification 2.Which guidelines proposed to method transfer
how can I make the copper get white bye any salt or acit?
about method developement in hplc
How we choose the mobile phase for method development.?
effect of pore size, pore volume, partical size, column length, carbon load on retention time? what is carbon load? what is the use?
How do we fix the sample concentaryion in hplc method development. What is the basis?
in each media we require to use SLS? how to proceed?
Describe your field experience sample type collected,sample techniques,field measurements taken and equipment used?