Interested to Buy Any Domain ? << Click Here >> for more details...
how calibrate the hplc detector
- 2 Answers
- 10599 Views
- Cipla, Shraddha Analytical Services, I also Faced
- E-Mail Answers
Answer Posted / m.sivalingeswara rao
Hplc detector calibration to be done to know the acuracy of
the emitted light wave length to the set value as well as
to check the sensitivity of the detecotr to respond with
the concentration of analyte.
To check accuracy generally caffeine solution is used
because it shows maxima and minima at particular wave
lengths(i.e maxima at 206nm,273nm and Minima at 241nm,in
this range we can check accuracy of lamp emitted light).
Sensitivity by permorming linearity test with different
concetration of caffeine solution.
Reproducibility by injecting the replicate injection of
Caffeine solution and measuring the %RSD of the responses
| Is This Answer Correct ? | 19 Yes | 0 No |
Post New Answer View All Answers
What is a difference between potency and purity?
What is the diffrence in japan mkt requirement in analytical method validation over US?
2. Two grams of Benzoic acid are dissolved in 200 ml of water and extracted with 200 ml of diethyl ether. The distribution coefficient of benzoic acid is 100, and its dissociation constant is 6.5 10-5. Calculate the distribution ratio (D) of benzoic acid at pH 2, 5, and 6. 3. Calculate D at pH 2 to 10 (1 unit apart) in the above problem, and plot D versus pH.
what parameters we will consider while developing a HPLC method and how we confirm our HPLC method is valid?
what is procedure of actual procedure for registration of drug?
What is the diference between residual solvents and organic volatile matter
Which products will produce when acetic anhydride reacts with Ter-butanol, Sec-butanol, Iso butanol, Dimethylamine etc.
what is mean by ambient temperature?
For titration in anhydrous media with perchloric acide, if lack of titrator, Which indicator is been used for replacement. How calculate pH of test solution to choose suitable indicator?
what is diffrence between specificity and selecivity?
how to selecet an exact coloumn for an new molecule development by hplc how to select exact salt as buffer for new molecule development by hplc what is the the process to select the mode of saparation of compoundes by hplc what is the use of ph of buffer what is use of buffer,ph,organic phase,ans methods how the molecules get saparated in coloumn,
how require to interprit the method precision data of hptlc?
how decide the clining method and cleaning method validation require for this perticular products?
what is the extinction coefficient for Indomethacin or at 319nm wave length.
What should be the level/ Percentage of Arsenic in Arsenic free zinc.
