In Number of Theoretical Plates (N) = 16 (tr/w)2.
where tr: retention time, and W: peak width.
My Question is here in formula what is 16.Can u explain
briefly?
---Veerabhadrarao.M
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Answer Posted / m.sivalingeswara rao
This belongs to statistical approach.Theoritical plates are
calculated by 4-Sigmamethod and 5-Sigma method.In 4-sigma
method the constant is 16 and in 5-sigma method constant is-
25
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