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I got 88.0 % assay for a drug in HPLC analysis for same
injection i got 40.0% related substances is it possible?
what is the relation between results of assay and purity?

Answer Posted / m.balaram naidu

PURITY IS QUALITATIVE ANALASIS
BUT ASAAY IS QUANTITATIVE ANALASIS.

EXP:-
CIPROFLOXACINE EXP
THIS PRODUCT PURITY IS 99.5% BUT ASSAY IS 99.8%
THE SAME PRODUCT PURITY IS 99.5% BUT ASSAY IS 80.0%
THE SAME PRODUCT PURITY IS 99.5% BUT ASSAY IS 50.0%
BECOUSE PURITY IS NOT DEPEND UP ON THE ASSAY.

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