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Why we use caffeine for HPLC calibration in Detector wave
ength accuracy test

Answer Posted / ravinder reddy

Caffeine is very stable compound and it is not degraded
it will give minimum and maximum wavelengths
it is easily soluble in methanol and acetonitrile (1 : 1)
it will get any c18 columns

Is This Answer Correct ?    5 Yes 2 No



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