Why we use caffeine for HPLC calibration in Detector wave
ength accuracy test
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Answer Posted / kondam.manoher reddy
caffeiene having different wavelenths it having 2 maximas
and 1 minima.
1st maxima is 205nm
2nd maxima is 273nm
minima is 245nm
and it is primary reference standard and also suggested in
pharmacopiea.it contains non recemic CHON.It is very stable,
we can use a long time for analysis
it is not degradable.
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