Why we use caffeine for HPLC calibration in Detector wave
ength accuracy test

Answer Posted / rajesh

caffeine is a universal primary standard and highly
stability nature.

Is This Answer Correct ?    6 Yes 1 No



Post New Answer       View All Answers


Please Help Members By Posting Answers For Below Questions

How doing qbd practically?

434


Why only hydroxy naphthol blue indicator is used for standardisation of 0.05M EDTA solution instead of solochrome Black T or Euriochrome Black T indicator which is used for all sample analysis with 0.05M EDTA solution?

2217


WHAT IS THE PARAMETER FOR THE SELECTION OF MOBILE PHASE IN HPLC?

652


what is mean by ambient temperature?

351


how to selecet an exact coloumn for an new molecule development by hplc how to select exact salt as buffer for new molecule development by hplc what is the the process to select the mode of saparation of compoundes by hplc what is the use of ph of buffer what is use of buffer,ph,organic phase,ans methods how the molecules get saparated in coloumn,

2046






mahagenco syllabus for the post of chemist

5744


Colorimtry

1178


In GC calibration,what is the procedure to calibrate the TCD (thermal counductivity detector)suggest ?

2717


What are usp limits for theoritical plats,resoution,tailing factor,peak to valley ratio

1344


some product having water content method with pyridine and ethylene glycol mixture instead of methanol? how they are selecting and methanol is not suitable solvent how ?

2102


using gradient pressure in gas chromatography are not ?using gradient pressure why

2943


AT WHAT CONDITIONS WILL YOU ANALYSE A SAMPLE,WHICH ARE PRESERVED AT OTHER THAN THE AMBIENT CONDITIONS?(ie COLD STORAGE SAMPLES BELOW 20* CENTIGRADE?

1607


2. Two grams of Benzoic acid are dissolved in 200 ml of water and extracted with 200 ml of diethyl ether. The distribution coefficient of benzoic acid is 100, and its dissociation constant is 6.5 10-5. Calculate the distribution ratio (D) of benzoic acid at pH 2, 5, and 6. 3. Calculate D at pH 2 to 10 (1 unit apart) in the above problem, and plot D versus pH.

1769


how you establish the LOQ?

315


How do we get end points and how many end points are possible for citric acid and di-acid not theorotically answer should be given practically.

2055