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Why forced degradation studies are performed in hplc method
validation?
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Answer Posted / sridhara h s
Evaluate stability of sample solution by repeatability in
one day (short time) or over days (long time).
Solution of sample must be stable for purpose of routine
analysis under normal laboratory conditions. Stability of
sample is important when a sample is under different
storage conditions.
Stability of sample needs special attention for gradient
analysis, requiring even 100 min or so. Mobile phase should
be selected to avoid stability problem.
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