Why we use caffeine for HPLC calibration in Detector wave
ength accuracy test
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Answer Posted / p.dharmakkannu
Caffeine basically having two maxima, lower wave length at
about 204nm and higher wave length at about 272nm. 1 minima
at about 242nm in methanol or mixture of methanol and water
combination(50%). It is nothing costly and easily soluble.
Maximum of the UV range products will be analyzed in this nm
range only.
it is also a primary reference standard officially in
pharmacopoeia for melting point.
If it is primary standard we will have certificate for the
caffeine. The same certificate will be used for both the
purpose.
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