what is the difference between transfection and
transduction?why the word trasfection is prefers for
transfer of genetic material through non viral particles in
eukaryote not for prokaryote??
Answer Posted / madhamanchi pradeep
Transfection: Transfer of naked DNA material into an
Eukariotic cell.
Transduction: Transfer of genetic material into Bacteria via
Bacterial viruses.
In case of prokareotes transfer of genetic material with out
any viral partical mediation is called TRANSFORMATION. But
in case of eukaryote this is called TRANSFECTION. It is
because Transfection of animal cells typically involves
opening transient pores or "holes" in the cell membrane, to
allow the uptake of material. Transfection can be carried
out using calcium phosphate, by electroporation, or by
mixing a cationic lipid with the material to produce
liposomes, which fuse with the cell membrane and deposit
their cargo inside.
Is This Answer Correct ? | 20 Yes | 12 No |
Post New Answer View All Answers
what are the shine-dalgarno sequences in organism other than E.coli.?
Why is it hard to keep genetically modified (GM) crops separate from non-GM crops throughout the manufacturing process?
Give an example for electrophilic substitution reaction?
Can you give me an example of a project you were involved with that illustrates your interest and skills in bringing people together?
How is this bio technology useful in our day to day life?
What do you mean by bio ethics?
Why do you use dbm instead of ncf in the northern blotting and what are the pre-treatments required for this paper to be used?
Sir i am doing my btech biotech at dy patil pune nd due to some prob i need to shift backbto surat so is it possible to transfer at bsc biotech college in surat
Hi i'm going to attend online test conducted by BCIL in few days...pls anyone can tell me about the test and how to prepare for it...
I completed my BSc in biotechnology and now in a dilemma what to choose next.My goal is to earn more so can anybody guide me the next step of my life which leads to my goal?
why the word trasfection is prefers for transfer of genetic material through non viral particles ineukaryote not for prokaryote?
in the lcmv what type of effect onm the mhc class i molecule
what is biological hydrogen production? 0 7 Hai friends, Can someone guide me..Am a postgraduate(m.tech) in biotechnology and am thinking of doing pg diploma course through distance mode. are they worth doing? if so which is better pg diploma in bioinfo or pg diploma in Intellectual property rights..am awaiting ur suggestions 0 20 hey i am doin bsc biotech and i want to do research on stem cells.....plz tell me how to go about?????? 0 32 in the lcmv what type of effect onm the mhc class i molecule 0 18 WHAT IS MEAN BY GENETIC ALGORITHM 1 56 Can I get a working model on petro plants ? 0 76 if i m doing bsc in biotechnology now.. are there any chances that i can do b.tech nw?? 0 59 what is multicolour biotechnology? what are the applications of multicolour biotechnology? 4 275 will i get f1 visa to pursue masters in US, if i have many backlogs and more than 2 yrs gap in btn my graduation. 0 85 suppose if u have gone through any biotech industry, what are the environmental conditions that u r expecting in quality control? Shanta-Biotech 2 598 suppose if u have gone through any biotech industry, what are the environmental conditions that u r expecting? Shanta-Biotech 1 261 what is the process of chees preparation 1 240 how biodiesel helps in reducing co2 1 313 what is multi colour bio technology? 1 200 i m doing bsc in biotechnology n i m confuse what to do in future...whether i shall do msc in biotechnology or any otyer course?in future which field will have greater oppurtunity?
How to isolate prokaryotic mRNA
What do you mean by bio-media?