Question { 35939 }

what is the difference between transfection and
transduction?why the word trasfection is prefers for
transfer of genetic material through non viral particles in
eukaryote not for prokaryote??

Answer

Transfection: Transfer of naked DNA material into an
Eukariotic cell.
Transduction: Transfer of genetic material into Bacteria via
Bacterial viruses.
In case of prokareotes transfer of genetic material with out
any viral partical mediation is called TRANSFORMATION. But
in case of eukaryote this is called TRANSFECTION. It is
because Transfection of animal cells typically involves
opening transient pores or "holes" in the cell membrane, to
allow the uptake of material. Transfection can be carried
out using calcium phosphate, by electroporation, or by
mixing a cationic lipid with the material to produce
liposomes, which fuse with the cell membrane and deposit
their cargo inside.