Interested to Buy Any Domain ? << Click Here >> for more details...
In my analysis by HPLC,In sample main peak and placebo
peak are eluting with shorter resolution ( almost look like
doublet). how can i get max resolution ? Is changing
gradient method is usefull?
- 3 Answers
- 12733 Views
- I also Faced
- E-Mail Answers
Answer Posted / wasim shaikh
You can try one or more of the following:
1) Changing the column type (c8 to c18, etc.)
2) Changing the Mobile phase composition (increase buffer
etc.)
3) Decrease the particle size on column (from 5 to 4, 3
etc.)
4) Increase the column length
5) Decrease the flow rate
6) If all of the above fails, then as you suggested,
gradient method can be used to separate the two peaks.
P.S. : Gradient method can also be used as an alternative
to the above methods and not necessarily the ultimate
solution.
| Is This Answer Correct ? | 32 Yes | 1 No |
Post New Answer View All Answers
Which is the highly polar and highly non polar column in HPLC?
which are the sizes of capsules?
HI,I CLEARED BOB CLERK EXAM. MY INTERVIEW WILL BE ON 9TH OCTOBER,2010.PLEASE SEND ME INTERVIEW QUESTIONS AND ANSWERS. THANK YOU.
What is third generation HPLC Columns?
Why acetonitrile and water are used as extraction solvent when analysis melamine? I thought they are miscible and won't be able to separate...
how require to set assay concentration for standard and sample?
please explian me about area normalisation method,diluted standard method,impurity vs impurity and which one has to be selected in method development
As per ICH related substances stability trend limit from initial to shelf life
using gradient pressure in gas chromatography are not ?using gradient pressure why
in sampling of raw material which side is pasted sampled labels
how much mass should be there in volumetric flask while in preparation of sample for assay?
what is mean by extactable and leachable study?
why we use glass fiber filters use in some situation?
How do we fix the sample concentaryion in hplc method development?
How to determine water content of bis tetrazole amine mono ammonium salt
