In my analysis by HPLC,In sample main peak and placebo
peak are eluting with shorter resolution ( almost look like
doublet). how can i get max resolution ? Is changing
gradient method is usefull?
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Answer Posted / wasim shaikh
You can try one or more of the following:
1) Changing the column type (c8 to c18, etc.)
2) Changing the Mobile phase composition (increase buffer
etc.)
3) Decrease the particle size on column (from 5 to 4, 3
etc.)
4) Increase the column length
5) Decrease the flow rate
6) If all of the above fails, then as you suggested,
gradient method can be used to separate the two peaks.
P.S. : Gradient method can also be used as an alternative
to the above methods and not necessarily the ultimate
solution.
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