How will decide to use HPLC OR GC for developing method for
unkown compund

Answer Posted / tushar

First check it is volatile or non volatile because HPLC is
for volatile or non volatile component and GC is for only
volatile component.After for HPLC check solubility of
component then v can use check UV , PDA , Refractive index
detecter.GC for FID,TCD,ECD detecter etc.

Is This Answer Correct ?    37 Yes 1 No



Post New Answer       View All Answers


Please Help Members By Posting Answers For Below Questions

What are the primary considerations in extractables and Leachables ?

1790


how you start the solubility study?

348


How do we fix the sample concentaryion in hplc method development. What is the basis?

682


inhouse product is in capsule form in combination and RLD is in tablet form then can we proceed for multimedia CDP? in inhouse capsule product disso is paddle with sinker in release media is there then RLD product in tablet form then with same as paddle with sinker we can proceed n.a.?

296


Why Ethanol is Used for Standardization of GC Head space?

3724






what is difference between UV - VISIBLE MODEL NO like 1600,1601,1700 etc ? plz explain me

2232


How to set analyticl specification for combination products?

308


CAN WE DETERMINE THE PEAK PURITY IN GAS CHROMATOGRAPHY ANALYSIS?

711


If combination product how require to identify which imp is of which api?

328


if rsd failed then what require to do?

328


Identify problem faced when mass spectroscopy is used with HPLC system ?

1389


How to decide assay range for non pharmacopeial API analysis by HPLC? Could you give me any reference for same e.g Guidelines or Paper publications?

2873


what is different when impergnated silica plates are used in separation of azo dyes using column chromatography?

1527


how you fix the limits of impurities?

387


is it nessesary to do solution stability for 7 days?

319