Why we use caffeine for HPLC calibration in Detector wave
ength accuracy test
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Answer Posted / eapen
Along with the above mentions
1.It can be seperated in any RP columns.
2.It is a highly polar compound.It elutes readily in a
reverse phase column. So less time is required.
3. It is highly stable in solutions for a longer time.
4. It is recommended to use Theobromine also in Detector
wavelength accuracy check.
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