what is method development parameter in HPLC method
development
Answer Posted / yojana
1. Check solubility,if sample dissolved in water then use
reverse phase,othervise use normal phase.
2.wavelength selection
3.selection of mobile phase,buffer.
4. pH OF BUFFER &MOBILE PHASE
5. selection of column
6. check retention time
7 .Flow rate
8. check system suitability parameter like
i)theoritical plates
ii) Assymetry
iii) Resolution
iv) capacitor fator
Is This Answer Correct ? | 31 Yes | 2 No |
Post New Answer View All Answers
using gradient pressure in gas chromatography are not ?using gradient pressure why
identification is for unknown? qualification for known? reporting for LOQ?
how to calibrate hplc & gc
If we have 5 strength which is not dose proportinate and excipients also diffrent in each strength then how we can proceed for Force degradation? and excipient are same but not dose propotinate the how FD?
i want model written test paper for the post of analyst in IOCL(indian oil corporation ltd) any one pls help me
How you develop a method in HPLC?
when we talk about change in entropy we consider total entropy means of surround +entropy of system but when we talk about change in Gibss free energy we consider entropy of system.explain with reason.
why octanol used to determine the partition coefficient ?
Why are use silicon oil mr?
As per ICH related substances stability trend limit from initial to shelf life
If vendor having more imp than monograph then how to proceed? and how to set spec?
Which parameters require to do in tech transfer?
How to choose the which salt is suitable for mobilephase
Why we select scan range from higer wavelength to lower wavelength in uv visible spectroscopy and ftir spectroscopy ? .
If combination product how require to identify which imp is of which api?