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what is method development parameter in HPLC method
development
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Answer Posted / yojana
1. Check solubility,if sample dissolved in water then use
reverse phase,othervise use normal phase.
2.wavelength selection
3.selection of mobile phase,buffer.
4. pH OF BUFFER &MOBILE PHASE
5. selection of column
6. check retention time
7 .Flow rate
8. check system suitability parameter like
i)theoritical plates
ii) Assymetry
iii) Resolution
iv) capacitor fator
| Is This Answer Correct ? | 31 Yes | 2 No |
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My question about gas chromatography sulfur chemiluminsecence detector. I test unknown sample gas by GC-SCD (calibrated ) and the result of *H2S is 279 PPM , *but when I test the same sample with the GC-TCD (calibrated ) the value of *H2S is 2500 PPM . I'd like to inform you that both GCs are calibrated and have very good operation conditions with stable parameters . the question is if the sample gas with higher H2S over detection limits of SCD detector (1000 ppm). why the result it 279 ppm Best regards
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