Answer Posted / ramana

Parameters involved during initial stage of Analytical
Method development:

1. Solubility
2. Pka Value
3. Selection of Buffers
4. pH of buffer/mobile phase
5. Composition of Mobile Phase
6. Selection of Column
7. Optimization of Analyte signal
8. Objectives of Seperation
8.1 Analysis time
8.2 Resolution
8.3 k factor (Capacity factor)
8.4 Peak height
8.5 Asyymetry
8.6 Theoritical Plates
9. Flow rate

1)What's the meaning of Absorption,give a example. 2)What's the meaning of Adsorption,give a example. 2)what is the difference between Absorption and Adsorption.

2876

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which one is better separation in high pressure liquide chromatogram orlowpressure chromato gram in hplc

2156

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how pda detector works over uv?

734

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Why are use silicon oil mr?

1191

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Ratio of Water and Acetonitrile(diluent) is not recommended to use in cleaning method validation,why?

2948

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How to decide assay range for non pharmacopeial API analysis by HPLC? Could you give me any reference for same e.g Guidelines or Paper publications?

3344

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sop of a uv visible spectrophotometer double beam elico model

5901

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which batch require to use for analytical method validation?

790

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what is the purge flow & how to calculate

2634

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how to develop the icp ms method? Application of icp ms?

800

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Is it nessesary all multimedia dissolution require descriminatory?

787

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[3/30, 13:29] Manoj P Venkatpurwar: How hplc column selection according to structure? How mobile phase buffer selection on molecule structure?

767

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[3/28, 20:52] Manoj P Venkatpurwar: how many impurities require to inject in assay specificity that how we can find out? and in Rs also how?

788

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if content uniformity passing but dissolution varrying then what is next step?

858

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in which situation ion pair require to use?

797

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