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Answer Posted / narasimha
Gas chramatography consist of Gas solid chramatography(GLC)
and Gas liquied chramatography (GLS). In both cases Gas are
used as Mobile phase and either Liquieds are solieds are
act as Statinary phase.
GAS SOLID CHRAMATOGRAPHY :Gas solid chramatography(GLC)not
widely used in pharamacitricul industery because of limited
number of statinary phases are available.The separation
principle is the adsorption in some cases Gas solid
chramatography is used if the solute is not soluble in
stationary phase that situations Gas solid chramatography
used
GAS LIQUIED CHRAMATOGRAPHY:Gas liquied chramatography(GLS)
Gas are used as Mobile phase Liquieds are act as Statinary
phase.Liquieds are coated on the solid support in the form
layer.the layer should be 0.5 to 1.0u
In gas chramatography process the components are convarted
into vapour stage and mixed with gaseous mobile phase.the
compound which is more soluble in stationary phase travels
slower and elluted later if the solute less soluble in
stationary phase travels faster and elluted out first. no
compounds have similar partition coefficient for fixed
combination of stationary phase, Mobile phase and other
conditions. Hence the compounds are separated accourding to
the partition coefficient
| Is This Answer Correct ? | 12 Yes | 4 No |
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