how do start hplc method development?
how much sample required for developing the method
development?
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Answer Posted / kizar ahamed
Method development starts with solubility of the
compound,if itis soluble in water v can try with reverse
phase if is is not v should use normal phase
chromatography.
2)selection of wavelength
3) column selection
4)Gradient setting(Always develope the method by using
gradient than isocratic)
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