Answer Posted / francois niyonsaba

To my knowledge, it is said that 2-ME is important for
lymphocyte or other cell cultures because the amino acid
cysteine is in short supply in FBS used in culture media,
while there is plenty of cystine. For example, T cells are
unable to transport cystine into the cytosol, where it must
be converted into cysteine. 2-ME reduces cystine into a
form that can be transported by the cells and is then
converted to cysteine needed for cell growth.
Another reason is that 2-ME, as a reducing agent, can break
down many of the toxic metabolites produced by cells in
culture thus improving the environment around the cells.


Francois NIYONSABA, M.D., Ph.D.
Associate Professor
Atopy/Allergy Research Center
Juntendo University School of Medicine
2-1-1 Hongo, Bunkyo-Ku
Tokyo 113-8421 JAPAN
Tel: +81-3-5802-1591
Fax: +81-3-3813-5512
E-mail: francois@juntendo.ac.jp

Is it possible for the transfection of primary neuronal cell cultures?

1818

---

Give an account of frozen plant tissue organelle isolation ?

1665

---

How does sodium thiosulphate affect yeast?

1774

---

What happens when the cell membrane or plasma membrane ruptures or breaks down?

852

---

Describe the electroporation condition ?

2006

---

What are cilia and flagella? How do these structures acquire movement?

902

---

What is a membrane?

880

---

What are Oral Keratinocytes ?

1864

---

what is the best way to set up the transfection?

1998

---

How do the rough endoplasmic reticulum and the Golgi apparatus act in the production and releasing of proteins?

902

---

Will the amount of G418 be affected by serum concentration?

1718

---

What is meant by suction force of the plant cell?

865

---

What would happen to photosynthesis if the green light waves that reach a vegetable were blocked?

1010

---

what is Sphere cells suspension culture ?

2002

---

Regarding FBS - What is the difference between the grades?

1891

---