Answer / francois niyonsaba

To my knowledge, it is said that 2-ME is important for
lymphocyte or other cell cultures because the amino acid
cysteine is in short supply in FBS used in culture media,
while there is plenty of cystine. For example, T cells are
unable to transport cystine into the cytosol, where it must
be converted into cysteine. 2-ME reduces cystine into a
form that can be transported by the cells and is then
converted to cysteine needed for cell growth.
Another reason is that 2-ME, as a reducing agent, can break
down many of the toxic metabolites produced by cells in
culture thus improving the environment around the cells.


Francois NIYONSABA, M.D., Ph.D.
Associate Professor
Atopy/Allergy Research Center
Juntendo University School of Medicine
2-1-1 Hongo, Bunkyo-Ku
Tokyo 113-8421 JAPAN
Tel: +81-3-5802-1591
Fax: +81-3-3813-5512
E-mail: francois@juntendo.ac.jp

Answer / chanchai

Beta-mercaptoethanol in cell culture medium was used to
differentiate from peripheral blood into neuronal cells

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