Question { 2376 }

what is the best method for detaching cells in culture ?

Answer

Trypsin(0.25%)+EDTA for 3min only at 37C

Question { 2648 }

What will happen to cell in 10X dilution RPMI1640+FBS ?

Answer

plasmolysis of cell occurs as u r using 10X(highly
concentrated) solution instead of 1X

Question { 3477 }

How to distinguish endo from exo cytosis in microscope?

Answer

Under microscope..
during endocytosis, u can observe pseudopodia formation and
invagination of the plasma membrane.
during exocytosis,the invagination (endosome) is found just
fused with membrane without pseudopodia formation

Question { 4496 }

what are Endosomes ?

Answer

endosomes are formed during phagocytosis (engulfing a
particle) by cells.

they usually fuse with lysosomes(which contain degradative
enzymes)and degrades/lyse the phagocytized particles.

Question { Cipla, 5201 }

in artificial respiration, why 5%carbon dioxide
uses with 95%oxygen?

Answer

Our tissues always contain CO2 in dissolved form as
bicarbonates (HCO3-). These are carried through RBC to lungs.

However, the cell works all the time & there is always
release of CO2 which is converted to bicarbonates & which is
also continuously transported to lungs.

So, if you consider at any time point, our body will have
that 5% CO2(bicarbonate form) inside as it is continuously
released. The rest (95%) is oxygen. Hence, in artificial
respiration 5% CO2 is also used such that it equals our body
conditions.

Question { 5217 }

what gas we inhale during night time

Answer

We inhale all available gases irrespective of their type at
all times (night or day).

But, we utilize only oxygen as our blood passing through
lungs has the ability to transport oxygen to other body parts.

Question { IIT, 8415 }

Why are the several carbohydrate side chains attached to
hydrophilic rather than hydrophobic regions of the membrane
protein ?

Answer

Carbohydrates acts as signalling molecules in cell. These
are bound by glycosidic bond to the membrane
protein(glycoproteins/proteoglycans) or lipid
molecules(glycolipids).

Carbohydrates choose hydrophilic amino acids of
transmembrane protein due to the fact that, they can easily
form glycosidic bonds with -OH group of hydrophilic aminoacids.
Whereas hydrophobic amino acids have complex structure with
usually no -OH groups for glycosidic bond formation.