Question { Avra, 11374 }

what is HPLC

Answer

high performance liquid chromatography

Question { 9355 }

What is Stray light ? & what is the IMP of this

Answer

Stray light is defined as the detected light of any wavelength that is outside the bandwidth of the
selected wavelength. The causes for stray light are scattering, higher order diffraction or poor
instrument design. Stray light causes a decrease in absorbance and reduces the linearity range of
the instrument. High absorbance measurements are more severely impacted by stray light.
Consider the follow scenarios at 1% transmittance level. If there is no stray light,
the absorbance value as governed by equation 1 should give a value of 2.