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Why is it necessary to cleave proteins with at least two
different reagents during sequencing?
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Answer / quido
generally the protein or polypeptide to be sequenced needs
to be cleaved into segments of size suitable or amenable to
the sequencing technique.
2 different reactions with two different cleaving agents is
used in order to allign the segments correctly once
sequencing is completed, with the help of overlapping
sequences, from the two digests.
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Micro Biology 
