what are the two imp charecters of nitrogenase complex?
Explain standard feat of formation.
explain henderson-hasselbalch equation?
How to name a glycosidic bond?
Explain entropy?
Mention the amino acids with positively charged R groups?
what are the two renal hormones?
if there are ester and aldehyde substituents on the pyridine ring . how I can oxidized the aldehyde substituent to carboxylic acid ? please help me
I have given the protocol for the cyclodextrin glygosyl transferase assay: One ml of appropriately diluted enzyme sample was incubated at 60 °C for 15 min with 5 ml of 1% (w/v) gelatinized soluble starch in 50 mM, 7.0-pH Tris–HCl buffer. Reaction was terminated by boiling the reaction mixture for 3 min and reaction volume was made to 10 ml with distilled water. Two ml of above reaction mixture was withdrawn and mixed with 3 ml of Tris–HCl buffer, 5 ml of 125 mM Na2CO3, and 0.5 ml of phenolphthalein (25 mg phenolphthalein/100 ml absolute alcohol). Absorbance was measured at 550 nm. The percent decrease of sample was calculated with respect to control containing 5 ml of buffer, 5 ml of sodium carbonate and 0.5 ml of phenolphthalein. where Acontrol = absorbance of control and Atest = absorbance of sample. The amount of β-cyclodextrin (β-CD) produced was estimated from the standard graph of 0–500 μg/ml β-CD concentration against % decrease in absorbance. One unit of CGTase was defined as the amount of enzyme required to produce 1 μm of β-CD/min. Please can you suggest me the formula for the defination given in the last line
why does separation not occurs in stacking gel in gel electrophorosis and why does separation done in separating gel?
what are the two types of methylmalonic acidemia(aciduria)?
How to measure average peptide chain length
what is lobry debrugn alberda van ekenstein transformation?