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why does separation not occurs in stacking gel in gel
electrophorosis and why does separation done in separating
gel?

Answers were Sorted based on User's Feedback



why does separation not occurs in stacking gel in gel electrophorosis and why does separation done..

Answer / biotech8

Stacking gel has a much larger pore size,and is merely used for stacking (arranging one over other) the samples so that they can be resolved in the separating gel easily. Since the separating gel has an optimal pore size so the samples separate by molecular sieving effect of the resolving gel which is absent in case of Stacking gel.

Is This Answer Correct ?    13 Yes 2 No

why does separation not occurs in stacking gel in gel electrophorosis and why does separation done..

Answer / guest

because pore size

Is This Answer Correct ?    4 Yes 1 No

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