What does the Iodine mordant do in a gram stain?
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Answer / sameer singh faujdar
By definition a mordant is a substance that set dyes. It doesn't really contribute to the colouring of the bacteria. In gram staining procedure iodine act as mordant by making crystal violet-iodine complex which actually retains in gram positive bacteria and not in gram negative bacteria, because of this complex gram positive bacteria appears violet in colour.
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Answer / biotrick.com
Iodine is used to fix the dye crystal violet
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Answer / laxmi kumari
it act as a mordant to stick crystal violet to the cell .
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Answer / bhupathi.e
Iodin enhances reaction between bacterial cell wall and crystal violet dye for giving violet colour
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