Interested to Buy Any Domain ? << Click Here >> for more details...
How to avoid contamination of primary cells during preparation?
- 2 Answers
- 4574 Views
- I also Faced
- E-Mail Answers
Answers were Sorted based on User's Feedback
Answer / anil kumar pr
The entire procedure of primary cell isolation and culture
should be carried out under strict aseptic condition as in
any cell culture practice. However, to minimize the chances
of contamination arising from the cell source such as
animal, organs or tissues, certain pretreatments are necessary.
1. As soon as the tissue is collected, place the it in PBS
(or any suitable buffer) containing high concentration
antibiotics (10X penicillin and streptomycin) for 2-5 min.
2. Immediately transfer the tissue to biosafety cabinet.
3. Rinse with buffer containing antibiotic (1X) (1-2 times).
4. Rinse with sterile PBS (3 - 5 times).
5. Transfer the tissue to buffer in a sterile vial and
remove all other items used for pretreatment from the
biosafety cabinet; such as waste buffer, tissue bits, used
pipette tips, container for waste collection etc.
6. Wipe the surface of BSC with 70% alcohol.
7. Wait for 2 minutes with 'flow' switched on.
8. Start cell isolation procedure.
| Is This Answer Correct ? | 0 Yes | 0 No |
Answer / n.c.pandey
Use of sterile wares,medium and use of Laminar flow hood
while preparing the primary cells.
| Is This Answer Correct ? | 0 Yes | 0 No |
what's the difference between 293FT and 293T?
What are the main components of the cytoskeleton?
How the chromosome region where the centromere is located is called? How are chromosomes classified in relation to the position of their centromere?
How does the intensity of facilitated diffusion vary in relation to the concentration of the moved substance? What is the limiting factor?
How do we determine if a hybridoma is producing antibody?
During what phase are many copies of a virus made and released?
Is there any need for linearize your vector before transfection?
what is the proper name for bottle holder thingee ?
Describe Bacteriophage growth?
Explain the Translocation and storage of Food ?
what are non-viral vectors?
What is osmotic pressure?
Micro Biology 
