How to avoid contamination of primary cells during preparation?
- 2 Answers
- 3645 Views
- I also Faced
- E-Mail Answers
Answers were Sorted based on User's Feedback
Answer / anil kumar pr
The entire procedure of primary cell isolation and culture
should be carried out under strict aseptic condition as in
any cell culture practice. However, to minimize the chances
of contamination arising from the cell source such as
animal, organs or tissues, certain pretreatments are necessary.
1. As soon as the tissue is collected, place the it in PBS
(or any suitable buffer) containing high concentration
antibiotics (10X penicillin and streptomycin) for 2-5 min.
2. Immediately transfer the tissue to biosafety cabinet.
3. Rinse with buffer containing antibiotic (1X) (1-2 times).
4. Rinse with sterile PBS (3 - 5 times).
5. Transfer the tissue to buffer in a sterile vial and
remove all other items used for pretreatment from the
biosafety cabinet; such as waste buffer, tissue bits, used
pipette tips, container for waste collection etc.
6. Wipe the surface of BSC with 70% alcohol.
7. Wait for 2 minutes with 'flow' switched on.
8. Start cell isolation procedure.
| Is This Answer Correct ? | 0 Yes | 0 No |
Answer / n.c.pandey
Use of sterile wares,medium and use of Laminar flow hood
while preparing the primary cells.
| Is This Answer Correct ? | 0 Yes | 0 No |
what is the role of L-glutamine in RPMI?
Of what substances is the nucleolus made? Is there a membrane around the nucleolus?
what are adherent cells ?
What is the biological term for programmed cell death?
What are cilia and flagella? How do these structures acquire movement?
How to stop proliferation?
WHO AMONG THE FOLLOWING DESCRIBED THE PROTOPLASM AS THE BASIS OF LIFE? A) TH HUXLEY B) LEEUWENHOEK C) RUDOLF VIRCHOW D) JC BOSE
What are the respective functions of phospholipids, proteins and carbohydrates of the cell membrane?
What is the way followed by the energy absorbed by plants to be used in photosynthesis?
fibroblast gives rise to myoblast . Myoblast gives rise to which structure?
Explain about PHOTOSYNTHESIS ?
what is the best cell culture media for in vivo imaging ?
Micro Biology 
