Describe the E. coli culture contamination ?
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Answer / priya
1.Culturing in the medium no 4 -endo
2.By microscoping - visible Lac "+"
3.Isolating them in the medium no 1 .
4.checking for biochemical activity
-medium with citrate, medium no 14 (should not be fermented)
-culturing in medium no 15 (formation of indol) indol "+"
-Cyt-oxidase "-"
- the sample contains Gr "-" bacteria, negative results in
oxidase, indol and does not utilise citrate.
-Conclusion contamination E-coli
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