How to use agar for antibiotic sensitivity testing ?
- 2 Answers
- 4759 Views
- I also Faced
- E-Mail Answers
Answers were Sorted based on User's Feedback
Answer / yasodha
Muller hinton agar is added to sterile water and
autoclaved.Thi is poured in to petri dishes to get 2 mm of
thickness under sterile conditions,These plates are stored
in refrigirater for storage.For sensitivity,make lawn
culture on media and keep antibiotic discs in radial
fashion at equal distance(not more than 6).Incubate at 37
degree centigrades for 24 hrs.So that sentivity zones
appear around the discs.Read the zone size with scale for
sensitivity.
| Is This Answer Correct ? | 3 Yes | 0 No |
Answer / shweta
For antibiotic sensitivity testing prepare a known
concerntration of agar medium by desolving it in sterile
d/w .After getting a transperent solution pour it in petri
dishes by maintaining a thikness of 2mm and let it set
down .Keep it in hot air oven for 1 min for removal of
moisture so that it does not creat any contamination. Take
the plates and place different antibiotics my maintaining
distance of 24 from each other. So in that way agar is used
for antibiotic sensitivity testing.
| Is This Answer Correct ? | 1 Yes | 3 No |
What is the function of a control group in an experiment?
What does liquescence digestive enzyme do?
What is a distilling flask?
Why is the reaction of ethane and chlorine not a free-radical addition reaction?
WHICH ANIMAL IS DUMB
How many different cranial nerves are there in the human body?
Which germ layer in mammals is predominantly responsible for forming the nervous system?
Order the following from the part of the digestive tract nearest to the beginning to the part nearest to the end: jejunum, ileum, esophagus, duodenum?
What is a graduated cylinder and how is it used?
What are the names and uses of the various laboratory tools?
Why do leaves change color?
What does acute disease mean?
Micro Biology 
