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Is coverslip needed after Gram stain?
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Answer / gud
coverslip is not neccesary or needed 4 viewing slid after
gram staining! thatz the correct ans!
| Is This Answer Correct ? | 15 Yes | 3 No |
Answer / jenna
Absolutely not. Focus on the low power and make your way
to high power, adding immersion oil as you flip
objectives. When you're finished and you want to keep the
slide just dab off the excess oil and place it in a slide
holder. Make sure you clean off the objective with some
lens paper. If none is available then blotting gently with
a Kimwipe will do. Just don't wipe with it because it
scratches easily. A coverslip CAN be used to make a
permanent slide, but it must be a glass coverslip applied
with fixative and allowed to dry. There are also some
(expensive) automated coverslip devices available for high
volume facilities since it can be a messy process.
| Is This Answer Correct ? | 2 Yes | 0 No |
Answer / shubhransu nayak
Using a coverslip after gram staining is difficult as we
have to observe it under 100x objective with immersion oil.
The coverslip sticks to the objective lense. But
immediately soak the objective lense with soft tissue paper.
| Is This Answer Correct ? | 1 Yes | 0 No |
Answer / ritchie
NO A COVER SLIP IS NOT NEEDED AS IT WOULD MAKE OBSERVATION
UNDER 100X VERY DIFFICULT A COVER SLIP IS USED FOR MOTILITY
TEST AND FOR FUNGAL IDENTIFICATION
| Is This Answer Correct ? | 0 Yes | 0 No |
Answer / bunty
If we want to observe under 100x object coverslip is needed.
but generally for Gram stained slide we observe under
10x/45x object.
| Is This Answer Correct ? | 2 Yes | 15 No |
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