How to select HPLC column for a particular product?
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In resolution test of uv calibration ratio of 269nm & 266nm, respectively, using hexane as the reference . the acceptance criteria for the ratio is NLT 1.5 but always found that 1.3 ,why what i can do for the resolving the problem is it necessary to integrate exat at 269nm and 266nm ehat is limit of tollerence
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