Explain the principle of ultracentrifuge?
Answer / biomaster
The ultracentrifuge method for determining the molecular
weights of proteins was developed by svedberg.
in this method protein molecules are subjected to
gravitational forces greater in magnitude than thermal
forces whereby causing them to diffuses.protein molecules
are larger in size called macromolecules.
under high centrifugal forces these molecules can be made
to sediment towards one end of a centrifuge tube.
The equation derived by svedberg for the molecular weight
of a protein is
M=RTS/D(1-Vp)
where R=gas constant
T=absolute temperature
s=sedimentation constant
D=diffusion constant
V=partial specific volume ofprotein
p=density of solvent
PRINCIPLE OF ULTRACENTRIFUGE IS "For most proteins however
the value of V is in the range of 0.70 to 0.75".
Is This Answer Correct ? | 53 Yes | 16 No |
How many elements are there in 3d-series of first transition series?
What is Chaemostat?
2 Answers Dr Reddys, Reddy Labs,
what are muco polysaccharides?
What are pentoses?
Describe the classification of all amino acids .
what is the wood sugar?
What do you like LEAST & MOST about your current position?
What are the structures of the products of this reaction, and how are they identified?
what are the two types of intermedins ?
what are the two imp charecters of nitrogenase complex?
why should i want to join your institute?
How much empty space is found in globular proteins?