Interested to Buy Any Domain ? << Click Here >> for more details...
What are "sticky ends". How are they formed?
- 4 Answers
- 23078 Views
- I also Faced
- E-Mail Answers
Answers were Sorted based on User's Feedback
Answer / sushma
The ends of the cut have an overhanging piece of single-
stranded DNA.
These are called "sticky ends" because they are able to
base pair with any DNA molecule containing the
complementary sticky end.
they r formed by restriction digestion using few restriction
enzymes
blunt ends can be be changed to sticky ends by addition of a
polylinker followed by restriction digestion
| Is This Answer Correct ? | 17 Yes | 3 No |
sticky ends are also called cohesive ends, it is formed by using endonuclease enzyme.
This enzyme will cut the strands in between so that it can produce those sticky ends or cohesive ends or overhanging ends
E.g 5'GAATTC 3'
3'CTTAAG 5'
Using EcoRI we can cut this strand.
| Is This Answer Correct ? | 11 Yes | 3 No |
Answer / mukesh kumar
dn a made polynucletide chain they are able to base pair the base in two strand are held together by hydrogen bond forming base pair with thymine one chain has 5 3 polrity while the other has 3 5
| Is This Answer Correct ? | 0 Yes | 3 No |
Answer / swapna
Recombinant DNA is DNA that has been created artificially.
DNA from two or more sources is incorporated into a single
recombinant molecule.
BamHI cuts the same site on both molecules
5' GGATCC 3'
3' CCTAGG 5'.
The ends of the cut have an overhanging piece of single-
stranded DNA.
These are called "sticky ends" because they are able to
base pair with any DNA molecule containing the
complementary sticky end.
In this both DNA preparations have complementary sticky
ends and thus can pair with each other when mixed.
DNA ligase covalently links the two into a molecule of
recombinant DNA.
| Is This Answer Correct ? | 4 Yes | 9 No |
What do you mean by bio ethics?
why the word trasfection is prefers for transfer of genetic material through non viral particles ineukaryote not for prokaryote?
What is the difference between traditional breeding and recombinant DNA techniques?
What does "determination of non-regulated status" mean?
how is beta propiolactone gets inactivated,please tell me the factors which affects its assay
2 Answers Biocon, Indian Immunologicals,
What are the possible benefits of the "Terminator technology"?
Size of Normal Land and bhosada
Cloning is a process where the nucleus of one egg is taken and it's coated with the membrane of other individual, to get a duplicate of a person. so my question is:when we use egg of some individual and membrane of some other how will we get a duplicate of a particular individual?
how my msc biotechnology degree is relevant to the niacl ao job
why methionin act as first aminoacid in protein synthesis?
wat should i do after m.sc.buotechnology
What is a Living Modified Organism (LMO)?
Micro Biology 
