What are "sticky ends". How are they formed?
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Answer / sushma
The ends of the cut have an overhanging piece of single-
stranded DNA.
These are called "sticky ends" because they are able to
base pair with any DNA molecule containing the
complementary sticky end.
they r formed by restriction digestion using few restriction
enzymes
blunt ends can be be changed to sticky ends by addition of a
polylinker followed by restriction digestion
| Is This Answer Correct ? | 17 Yes | 3 No |
sticky ends are also called cohesive ends, it is formed by using endonuclease enzyme.
This enzyme will cut the strands in between so that it can produce those sticky ends or cohesive ends or overhanging ends
E.g 5'GAATTC 3'
3'CTTAAG 5'
Using EcoRI we can cut this strand.
| Is This Answer Correct ? | 11 Yes | 3 No |
Answer / mukesh kumar
dn a made polynucletide chain they are able to base pair the base in two strand are held together by hydrogen bond forming base pair with thymine one chain has 5 3 polrity while the other has 3 5
| Is This Answer Correct ? | 0 Yes | 3 No |
Answer / swapna
Recombinant DNA is DNA that has been created artificially.
DNA from two or more sources is incorporated into a single
recombinant molecule.
BamHI cuts the same site on both molecules
5' GGATCC 3'
3' CCTAGG 5'.
The ends of the cut have an overhanging piece of single-
stranded DNA.
These are called "sticky ends" because they are able to
base pair with any DNA molecule containing the
complementary sticky end.
In this both DNA preparations have complementary sticky
ends and thus can pair with each other when mixed.
DNA ligase covalently links the two into a molecule of
recombinant DNA.
| Is This Answer Correct ? | 4 Yes | 9 No |
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