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what laboratory procedures are followed when working with
microorganisms?
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Answer Posted / chetali
1. To minimise the risk of spreading microbial
contamination, you should have a laboratory coat that you
use only for microbiology work.
2. All cuts or abrasions should be protected with
waterproof plasters before starting work. Hands must be
disinfected or washed immediately when contamination is
suspected, after handling infective materials and also
before leaving the laboratory (even if only leaving for a
short time) using soap and warm water at the wash-hand
basins provided.
3. The laboratory door must be closed when work is in
progress. Bench surfaces must be disinfected beforehand.
4. No work should commence unless adequate discard
facilities, appropriate to the work being undertaken, are
available.
5. Use all sterilized glassware, equipment and medias to
avoid any contamination.
6. Sterilise all the waste cultural medias in an autoclave
before disposal.
7. Label each and every item.
8. Tidy up after use, do not leave any unidentified
cultures, solutions etc. on the bench, and decontaminate
the work surface with a disinfectant-soaked mop followed by
drying with paper towel before leaving the laboratory.
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