what are the steps involved in the formation of osazone
from glucose?

Answer Posted / gaurav

The first hydrazine molecule forms a condensation product
with aldehyde or ketone group, and while doing that forms a
cyclic H bonded intermediate with the hydroxy ("H" of the
adjacent -OH,what follows is the transfer of hydrogen on the
carbon bound to N and thus oxidizing the first carbon
[initial -OH bearing carbon]. The second hydrazine abstracts
one proton from the aldehyde and gets reduced to aniline and
ammonia and thus oxidizes aldehyde to ketone,wherein you
require the third phenyl hydrazine molecule to complete the
Osazone formation :)

For more information check out
"Barry & Mitchell - Nature 175, 220 [29 Jan 1955]

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