Interested to Buy Any Domain ? << Click Here >> for more details...
Answer Posted / vinee
DNA shuffling(Sexual PCR), in contrast, is PCR without
synthetic primers.
In this process, a family of related genes--say, the ones
that codes for the surface proteins of three different HIV
isolates--are first chopped up with enzymes.
The gene fragments then are heated up to separate them into
single-stranded templates. Some of these fragments will
bind to other fragments that share complementary DNA
regions, which in some cases will be from other family
members.
Regions of DNA that are non-complementary hang over the
ends of the templates (see illustration). The PCR reaction
then treats the complementary regions as primers and builds
the new double-helical DNA.
But PCR also adds bases to the overhanging piece of the
primer, forming a double helix there, too. This ultimately
creates a mixed structure or chimera. In the final step,
PCR reassembles these chimeras into full-length, shuffled
genes.
| Is This Answer Correct ? | 1 Yes | 0 No |
Post New Answer View All Answers
What is the Transposon for STM ?
Why did you decide to study microbiology? What do you like about it?
Give an account of glycerol sterilisation ?
Contrast the streak plate isolation and pour-plate isolation methods for making population measurements?
Why are the bacilli arranged at angles to each other?
How to grow more methylobacterium?
Describe Metal Cylinder Diffusion Method ?
Describe Shigella flexneri ?
How to measure bacteria?
How microorganisms vary in pathogenesity?
What is meant by fungal element?
How to extract hydrophobic proteins from yeast wall cells?
Give an acccount of aeromonas ?
what are the microbes that grow if proper temperatures are not maintained during fermentation process?
Give an account of removal of and microscopic visualization of extracellular matrix?
