What reagents can be used to break disulfide bonds?
- 10 Answers
- 28118 Views
- I also Faced
- E-Mail Answers
Answer Posted / g.rajesh kumar
Mercaptoethanol breaks the disulphide bonds in proteins and
sds makes the protein to possess negative charge, so that
proteins can be separated based on their charge in sds-page
chromatography.
| Is This Answer Correct ? | 23 Yes | 1 No |
Post New Answer View All Answers
Which univalent element cannot form alums and why?
What are the important sources of phosphorus?
Why are right-handed helices more stable than left-handed helices?
Which electrode acts as reference electrode and gives its potential value?
Why is the t-butyloxycarbonyl protecting group such a good choice for amino acid synthesis?
Why can it be said that the enzymatic action is highly specific?
Aldehydes and ketones characteristically undergo which reactions?
What does one Debye equals?
What are some factors that can lead to protein denaturizing?
What is the tertiary structure of a protein? What are the main types of tertiary structure?
Calculate the molar extinction coefficient of a solution containing 5 *10-4 g litre-1 of a biomolecule, molecular weight 275 g mol-1, and absorbance 0.75 in a 1.2 cm cuvette.
What is the Chemical formula for Epsom salt?
Since pepsin is a gastric enzyme does it, has acid or basic optimum ph? What happen to pepsin when it passes to the duodenum?
What are pentoses?
What are hydrophobicity scales, and how are they used?
