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How do I grow hybridoma cell lines as ascites, especially
rat-mouse hybridomas?
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Answer Posted / guest
ATCC propagates most hybridomas as cell cultures in vitro,
but many can be grown efficiently as ascites by inoculation
into the peritoneal cavities of mice. Some rat-mouse
hybridomas are more problematical as the host mouse strain
may develop anti-rat antibodies and eventually reject the
hybridoma inoculum. To minimize this difficulty one can use
athymic nude mice as hosts or immunosuppress host animals
prior to inoculation
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