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*PI (Population Doublings, PD):*
Population Doublings (PI) measures the number of times a cell population doubles in number. It's calculated by:
PI = log2 (final cell number / initial cell number)
PI helps track cell growth, proliferation, and senescence. It's essential in cell culture, particularly for:
1. Cell line characterization
2. Aging studies
3. Cancer research
4. Cellular therapy development
*Human Lymphocyte Cultures:*
Human lymphocyte cultures involve growing immune cells (lymphocytes) in the laboratory. These cultures are crucial for:
1. Immunological research
2. Cancer studies
3. Vaccine development
4. Gene therapy
5. Diagnostic testing
Types of lymphocytes cultured:
1. B cells
2. T cells
3. Natural Killer (NK) cells
Culture methods:
1. Peripheral Blood Lymphocytes (PBLs)
2. Lymphoblastoid cell lines (e.g., EBV-transformed)
3. Primary lymphocyte cultures
4. Immortalized lymphocyte cell lines
Applications:
1. Immunophenotyping
2. Functional assays (e.g., cytotoxicity, proliferation)
3. Gene expression analysis
4. Cell-based therapies
*Key aspects of lymphocyte cultures:*
1. Media: RPMI 1640, DMEM, or specialized media
2. Growth factors: IL-2, IL-7, or other cytokines
3. Stimulators: Mitogens (e.g., PHA), antigens, or antibodies
4. Cell separation: Density gradient centrifugation or magnetic bead separation
*Challenges and considerations:*
1. Cell viability and proliferation
2. Contamination risk
3. Immortalization and transformation
4. Ethical concerns (e.g., donor consent)
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