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What is the exact reason to use THF and IPA in buffers of
Reverse phase HPLC as a solvent?
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Answer Posted / bhushan patil.
Both THF and IPA are modreatly polar solvent dissolved most of the polar and nonpolar compound .In revers phase tecnique stationary phase is Non-polar and mobile phase is polar.Hence this solvent are used in revers phase.
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