What is the exact reason to use THF and IPA in buffers of
Reverse phase HPLC as a solvent?
- 1 Answers
- 4126 Views
- Dr Reddys, I also Faced
- E-Mail Answers
Answer Posted / bhushan patil.
Both THF and IPA are modreatly polar solvent dissolved most of the polar and nonpolar compound .In revers phase tecnique stationary phase is Non-polar and mobile phase is polar.Hence this solvent are used in revers phase.
| Is This Answer Correct ? | 0 Yes | 1 No |
Post New Answer View All Answers
For standardization of volumetric solutions, acceptance limit for concentration is considered as 10% of nominal concentration and %RSD as NMT 0.2% (for appropriate no. of replicates). But , if %RSD has not met, what should be further action?
what is mean by dissolution hydrodynamics?
How can the GC or HPLC method is selected to determine the impurity profile in drug product?
How would you decide dissolution medium for NCE compound of class I drug
If change in specification which parameter require to do for validation? if change in chromtographic condition then which parameter? if api change then which parameter? if change composition then which parameter? if old method not work out then whicj parameter? if additional one impurity added then which parameter of validation require to do on above each conditions? elaborate separately
what is mean by nitrosamine impurities?
What is the principle of HPLC, GC, LCMS, GCMS,LC QQQ, GC QQQ, LC Q TOF and GC Q TOF. What are the applications and Specificity?
in each media we require to use SLS? how to proceed?
UV and PDA detector, which have less signal to noise ratio?
What is the requirement for brazil in the analytical method vslidation comparr to ich?
Which is the highly polar and highly non polar column in HPLC?
How to determine the EDTA content by potentiometry titration in Ceftriaxine sodium
WHY given much noise PDA dector then VWD Dector
what is mean by dissolution biowaiver study?
Why dissolution test is not performed in all of the products
