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Answer Posted / manoj reddy
PDA monitors the absorbance over a wide range of wavelengths, while UV detector monitors the UV absorbance at a selected wavelength. The diode array element measures the intensity of light at each wavelength.
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please explian me about area normalisation method,diluted standard method,impurity vs impurity and which one has to be selected in method development
in OSD forms require to use gas chromatography?
Why to use propyl paraben in HPLC gradient calibration
From where require to take the RLD sample?
what is the purge flow & how to calculate
when we talk about change in entropy we consider total entropy means of surround +entropy of system but when we talk about change in Gibss free energy we consider entropy of system.explain with reason.
how to calibrate hplc & gc
2. Two grams of Benzoic acid are dissolved in 200 ml of water and extracted with 200 ml of diethyl ether. The distribution coefficient of benzoic acid is 100, and its dissociation constant is 6.5 10-5. Calculate the distribution ratio (D) of benzoic acid at pH 2, 5, and 6. 3. Calculate D at pH 2 to 10 (1 unit apart) in the above problem, and plot D versus pH.
How require to perform linearity as per ANVISA? What are the acceptance criteria?
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Basic principle of ESI?
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In the HPLC Calibration done wavelength accuracy done between 200nm-280nm .but not done remaining 300-400nm not done ?
I have compare C2H2-air and C2H2-N2O flame AAS on determination calcium. Both use same range of std to plot calibration curve. (2-6ppm) When i measure the sample with phosphate, KCl and LaCl, C2H2-N2O flame give false positive result, around 0.5ppm. When i measure the sample with phosphste, KCl and EDTA. C2H2-N2O flame also give 0.5ppm false positive. But both above mentioned sample would not give false positive when measured by C2H2-air flame. What is the reason?
