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Dear sir, This is R.Kadambavadani working in a pharma industry. while doing RS analysis of ibuprofen API, unknown peak eluting some times in the 0.6 Min RT. Kindly send me what is the exact reason behind that. also am using ACN the mobile phase.

Answer Posted / nikkosthiru@gmail.corai12

Please mention what is your diluent?
Whether used new HPLC vial or old?
The peak eluted for void volume please ensure what r the chemical used in this test method

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