What are the factors effect peak shape in HPLC ?
Answer Posted / chan
It depends on column efficiency, pH of buffer and column temperature.
| Is This Answer Correct ? | 0 Yes | 0 No |
Post New Answer View All Answers
What is similarity factor and its use in api strength?
What is dose dumping? why require to do?
What should be the level/ Percentage of Arsenic in Arsenic free zinc.
How to fix the sample conc. in Related substances test by HPLC?
what is the principle involved in turbidometry and nephelometry ?
What is the formula for relative diffrence for standard solution in solution stability in validation?
If inhouse hplc related substance method is completly diffrent from Usp for finished proďuct with diffrent impurities then how require to prove method equivalecy?
in hplc chromatogram started from left to right and in uv spectrum started from left to right
if tech transfer fails whay require to do?
What is a difference between potency and purity?
why are measure gas flow " ml " in Gas chromatography
What is column in chromatography?
Why to use propyl paraben in HPLC gradient calibration
2. Two grams of Benzoic acid are dissolved in 200 ml of water and extracted with 200 ml of diethyl ether. The distribution coefficient of benzoic acid is 100, and its dissociation constant is 6.5 10-5. Calculate the distribution ratio (D) of benzoic acid at pH 2, 5, and 6. 3. Calculate D at pH 2 to 10 (1 unit apart) in the above problem, and plot D versus pH.
Loss of linearity in tkn analysis, possible reasons?