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Answer Posted / r.kadambavadani
The column stationary phase is also affected by pH. At very low pH (<2) the bonded stationary phase will be stripped from the silica support. At high pH (>8) the silica itself will be damaged by dissolution. At mid pH ranges (>5), any lone silanol groups (Si-O-H) present will act like an acidic moiety and will become ionized - increasing the tailing observed with analytes containing basic functional groups.
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